基因編號: NM_001220475
產品編號: CH801379
基因名稱: PPFIA2
基因大小: 3711 bp
分子量: 136.07 kDa
酶切位點: SgfI_MluI
價格: 詢(xun)價
供貨時間: 電詢 400-077-2566
產(chan)品(pin)名稱(cheng)：人源cDNA克隆
產品(pin)規格(ge)：500μL甘油菌液
產(chan)品運輸(shu)條件：常溫
產品儲存條(tiao)件和期(qi)限(xian)：-80℃長期保存(cun)
產品特(te)點：
? 人源基因C-端融合 Flag & 6×His 標(biao)簽；
? 含有puromycin真核抗(kang)性基因，可篩選穩定轉染細胞株；
? 含有卡那霉素(su)原核(he)抗性基因(yin)，可(ke)篩選重組克隆，推薦使用濃度為30μg/ml。
產品(pin)使用：
將甘(gan)油菌在新鮮配制的(de)固體培養基（相應抗性(xing)）中劃線，37℃培(pei)(pei)養(yang)(yang)箱培(pei)(pei)養(yang)(yang)過(guo)夜，挑取(qu)3個(ge)單克隆，分別接種(zhong)于相(xiang)應抗(kang)性的液體(ti)培(pei)(pei)養(yang)(yang)基中(zhong)，37℃搖床培(pei)(pei)養(yang)(yang)過(guo)夜，然后進行質粒的提取(qu)和驗證。
溫馨提示：考(kao)慮到實(shi)驗的嚴謹性，建議(yi)您(nin)拿到產品的第一時間進(jin)行測序確認。
ATGATGTGTGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAAAGGGGGTCCCAAAGCAGTGGCTCGGACTCAGACTCCCATTTTGAGCAGCTGATGGTGAATATGCTAGATGAAAGGGATCGTCTTCTAGACACCCTTCGGGAGACCCAGGAAAGCCTCTCACTTGCCCAGCAAAGACTTCAGGATGTCATCTATGACCGAGACTCACTCCAGAGACAGCTCAATTCAGCCCTGCCACAGGATATCGAATCCCTAACAGGAGGGCTGGCTGGTTCTAAGGGGGCTGATCCACCGGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACTTCTAGAAAAGGAAGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCATTTGGAGTGCCTTGTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTCCCTCAGGAGTATCCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTTGGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAAGAACTAGCTGCTGCTAATCAGGAGATTGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCGAGGGATCCACAGAGTCAGAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTCCAATGGTTCTATAGACTCAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAAAACTATGAAATGGCCCAGATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGGAAGCAGAGACAGCAAGAAAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAGGGAGGCCATGGCACAAAAGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCTCAGAGAGAATCTACCTCCATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTATCCTGCGGCAGATGGAAGAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCAGCAGACCATGAGAAAGGCTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTAACCAAGGCTGAAGAGAGACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGAAGAATCAAGAACTTCAAAGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGATACGGTTGATAGACTTCTGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCTCTAGAAGAAAAGAATGTTTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATGATAAGGAAAGATTAGCAGAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGGCTCTTTAATTGAACCCACAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGATCCCTAGTGGACAGCCAGTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGGGTGTGCGAAGAGATGAGCCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGGAGTACTAAGCAGCCACCCTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACAATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTCAGGAACAATTGGATGCCATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCGTGCTGAAGAAATTGAAAATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCAGGTACCTCCATTACTGCCTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAACTCCAAAGCTCACCCCTCGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGATCTGAGGAAACATCGGAGAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAATGTGAAACTTCTCCTCCTCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACAATGATGCTCGAAGTAGTTTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAGCCAAGACTCTCTTCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAAAAAGAAAAAGCTCGACTTGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGGGGTTAGGCAAACTCGGAACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGAAGCTCGGAGAAAGGGATTACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGGTTGGGAATGCCTGCGTGGTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTTTATCTGACACTGAGATCCAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGCAATCCAGGAGATGGTTTCCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTAAAGAATCTGAGGAAGGAAGCTGGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAATCATGAGTGGATTGGAAATGAATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAAGTTACTTTATGGAATGCTTGGTAGATGCAAGAATGTTAGATCACCTAACAAAAAAAGATCTCCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAATATGGAATTATGTGCTTAAAGAGGTTGAATTATGACAGAAAAGAACTAGAAAGAAGACGGGAAGCAAGCCAACATGAAATAAAAGACGTGTTGGTGTGGAGCAATGACCGAGTTATTCGCTGGATACAAGCAATTGGACTTCGAGAATATGCAAATAATATACTTGAGAGCGGTGTGCATGGCTCACTTATAGCCCTGGATGAAAACTTTGACTACAGCAGCTTAGCTTTATTATTACAGATTCCAACACAGAACACCCAGGCAAGGCAGATTCTTGAAAGAGAATACAATAACCTCTTGGCCCTGGGAACTGAAAGGCGACTGGATGAAAGTGATGACAAGAACTTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCCTCCTCGTGAAGTACATGGAATCAGCATGATGCCTGGGTCCTCAGAAACATTACCAGCTGGATTTAGGTTAACCACAACCTCTGGGCAGTCAAGAAAAATGACAACAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGT
MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDRDSLQRQLNSALPQDIESLTGGLAGSKGADPPEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELAAANQEIVALREQNVHIQRKMASSEGSTESEHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQELLEKQNYEMAQMKERLAALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEEKNVLIQESETFRKNLEESLHDKERLAEEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVGSLVDSQSDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDIDDDDRETIFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPPTPRALRMTHTLPSSYHNDARSSLSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGKKEKARLGQLRGFMETEAAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTKESEEGSWAQCPVFLQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDRKELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYANNILESGVHGSLIALDENFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGSTWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC
維真生物的人源cDNA克隆中，雖然我們大約70%的(de)克隆與RefSeq或GeneBank所發表(biao)的基因列(lie)序完全一致,但也有(you)些克隆含有(you)天然(ran)的單核苷酸多態性而包含一至多個SNPs, 因此與RefSeq或GeneBank所發表的列序(xu)會有些不(bu)同(tong)。我們(men)強烈建(jian)議您在購買之(zhi)前仔細將我們(men)克隆的序列(lie)與您所要求的序列(lie)進行對比，以決定(ding)所(suo)購買的(de)克(ke)隆是否符(fu)合您的(de)要(yao)求(qiu)。如果我們預(yu)制(zhi)的(de)克(ke)隆不符(fu)合您的(de)要(yao)求(qiu)，我們一般在1-2周內可(ke)以提供一個與您所(suo)要(yao)求的(de)序列完全(quan)一致的(de)克隆。我們的(de)每一個克隆與RefSeq（2013）標準(zhun)序列相比都含有一(yi)個完整(zheng)的ORF(Opening Reading Frame)。因(yin)為RefSeq數據庫(ku)會不斷更新，更新后的(de)(de)標準序列(lie)將來有(you)(you)可能與我們(men)現(xian)有(you)(you)的(de)(de)克隆序列(lie)產(chan)生(sheng)差異(yi)。我們(men)所有(you)(you)的(de)(de)克隆產(chan)品都經過二代（NextGen）測序(xu)驗證(zheng)，并且保證(zheng)網站上(shang)克(ke)(ke)隆的序(xu)列與您收到的克(ke)(ke)隆序(xu)列完全一(yi)致(zhi)。在未經(jing)維(wei)真生物書(shu)面給予授權前，人源ORF cDNA克隆不可轉售或(huo)贈予買(mai)方以外的(de)第三方。同時, 在使用任何(he)維真產品之前，買(mai)方有責任(ren)確認該基因的使(shi)用(yong)不會(hui)侵(qin)犯到第三方的知識產權。此產品(pin)專門為(wei)基礎(chu)研究所開發，不適用(yong)于(yu)任(ren)何治療或診斷應用(yong)。
400-077-2566
service@wzbio.cn
