產品名稱(cheng):人(ren)源cDNA克隆(long)
產品規格:500μL甘(gan)油菌液(ye)
產(chan)品(pin)運輸條件:常溫(wen)
產(chan)品儲存條件和(he)期限:-80℃長期保存
產品特點:
? 人(ren)源基因C-端融合 Flag & 6×His 標簽;
? 含有puromycin真(zhen)核(he)抗性(xing)基(ji)因,可篩(shai)選穩定轉染細胞(bao)株;
? 含有卡那霉素原核抗性基因,可篩選重組克隆(long),推薦使用濃度為(wei)30μg/ml。
產品(pin)使用:
將(jiang)甘油菌在新鮮(xian)配制(zhi)的固體(ti)培養基(相應抗性)中劃線,37℃培養(yang)箱培養(yang)過(guo)夜(ye)(ye),挑取(qu)3個單克隆(long),分(fen)別接種于相應抗性的液體培養(yang)基中,37℃搖(yao)床培養(yang)過(guo)夜(ye)(ye),然(ran)后進行(xing)質粒的提取(qu)和(he)驗證(zheng)。
溫(wen)馨(xin)提(ti)示:考慮到實(shi)驗的(de)嚴謹(jin)性,建議您拿到(dao)產品的第一時間(jian)進行測(ce)序(xu)確認。
CGCCATGAGGGTCCCTGTATTTGAGGATATAAAAGATGAAACTGAAGAAGAAAAGATAGGGGAAGAAGAAAATGAAGAAGACCAGGTCTTCTATAAGCCTGTTATTGAAGACTTAAGCATGGAATTGGCCAGAAAATGCACGGAACTCATTAGCGATATCCGTTATAAAGAAGAGTTTAAAAAGTCCAAGGATAAGTGTACATTTGTGACTGACAGTCCTATGCTAAACCATGTAAAAAATATCGGTGCTTTTATTTCTGAGGCAAAATACAAAGGCACCATTAAAGCTGACCTTTCTAATTCTCTTTATAAGCGGATGCCAGCCACAATTGACAGTGTTTTTGCAGGAGAAGTTACACAGCTCCAGAGTGAGGTGGCCTACAAGCAGAAACATGATGCTGCCAAAGGATTCTCAGATTATGCCCACATGAAGGAGCCCCCTGAGGTTAAACATGCCATGGAGGTCAATAAACACCAGAGTAATATTTCTTATAGGAAAGACGTGCAGGACACCCACACGTACAGTGCAGAACTTGACCGACCAGACATCAAGATGGCAACCCAGATCTCTAAGATCATAAGCAATGCAGAATACAAGAAAGGACAAGGAATAATGAATAAAGAGCCCGCTGTAATTGGAAGACCAGATTTTGAACATGCCGTGGAAGCTTCTAAACTTTCTAGTCAAATTAAATACAAAGAAAAATTTGATAATGAAATGAAGGATAAGAAACATCATTACAATCCTCTTGAAAGTGCTTCTTTTAGGCAGAATCAGCTTGCTGCTACACTGGCGAGCAATGTGAAGTACAAGAAAGACATTCAAAATATGCATGATCCAGTTTCAGATCTCCCAAATTTGTTGTTTTTAGACCATGTTTTGAAAGCCAGCAAAATGCTCAGCGGCCGAGAATATAAAAAGCTCTTTGAGGAAAACAAAGGAATGTATCATTTTGATGCAGATGCTGTGGAACATCTGCACCataaaggcaatgcCGTCCTCCAAAGTCAGGTGAAATATAAAGAAGAATATGAGAAAAATAAGGGAAAGCCAATGCTTGAATTTGTTGAGACACCATCATATCAAGCTTCAAAGGAGGCTCAAAAGATGCAAAGTGAAAAAGTTTACAAAGAGGATTTTGAGAAGGAGATTAAAGGAAGGTCATCACTGGATTTAGACAAGACTCCAGAATTTTTACATGTAAAGTACATCACCAACCTTCTGAGGGAGAAAGAATATAAAAAAGATTTGGAAAATGAGATAAAAGGGAAAGGAATGGAACTTAATTCAGAAGTTCTTGATATCCAAAGAGCAAAGCGAGCCTCTGAAATGGCAAGTGAGAAAGAATACAAGAAAGACCTGGAGTCAATAATTAAAGGGAAAGGAATGCAAGCTGGCACTGACACCCTTGAAATGCAGCATGCCAAGAAGGCTGCAGAGATAGCGAGTGAGAAAGACTATAAAAGAGATCTGGAGACTGAAATTAAAGGGAAAGGGATGCAGGTGAGCACAGACACTCTTGATGTCCAGAGAGCTAAGAAAGCATCCGAGATGGCCAGCCAGAAACAATACAAGAAGGACTTAGAAAATGAAATTAAAGGGAAAGGAATGCAAGTGAGCATGGATATCCCAGATATCCttcgagccaagaGGACATCTGAAATCTATAGCCAGAGAAAGTATAAAGATGAAGCAGAGAAGATGCTTTCTAACTATTCTACCATAGCAGATACTCCTGAAATTCAGAGAATTAAGACAACTCAACAAAACATTAGTGCGGTATTTTATAAGAAAGAAGTGGGAGCTGGCACTGCAGTGAAAGATAGCCCAGAGATCGAACGAGTGAAGAAAAATCAGCAGAATATTAGTTCAGTGAAATACAAAGAAGAGATTAAACATGCAACAGCCATTTCTGATCCTCCAGAACTAAAGAGAGTTAAAGAAAACCAGAAGAACATCAGCAATCTCCAGTATAAAGAGCAAAACTACAAGGCCACTCCGGTAAGCATGACCCCGGAGATAGAGAGAGTGAGGCGAAACCAGGAGCAGCTGAGTGCGGTAAAATATAAGGGAGAACTTCAACGGGGAACTGCAATTTCTGATCCACCAGAGCTGAAGAGGGCAAAAGAAAACCAGAAAAACATCAGCAATGTTTATTACAGAGGTCAGCTGGGAAGAGCTACCACTTTAAGTGTAACTCCTGAAATGGAAAGAGTGAAGAAGAATCAAGAAAATattagctcggtAAAATATACCCAGGACCATAAACAGATGAAAGGTAGACCAAGTCTGATTTTAGATACACCTGCTATGAGACATGTTAAAGAAGCACAAAATCATATTTCAATGGTAAAATACCATGAAGATTTTGAAAAAACAAAGGGGAGAGGCTTTACTCCCGTCGTGGACGATCCTGTGACAGAGAGAGTGAGGAAGAACACCCAGGTGGTCAGCGATGCTGCCTATAAAGGGGTCCACCCTCACATCGTGGAGATGGACAGGAGACCTGGAATCATTGTTGACCTCAAAGTTTGGCGCACAGATCCTGGCTCCATCTTCGACCTTGATCCCCTGGAAGACAATATTCAGTCTAGAAGTCTCCATATGCTCTCTGAAAAGGCGAGTCACTATAGGCGACACTGGTCTCGATCCCATTCCAGCAGTACTTTCGGTACAGGTCTCGGAGACGACAGgtcagaaatctccgAGATTTACCCTAGCTTTTCATGCTGCAGTGAGGTAACAAGACCGTCTGATGAAGGAGCACCTGTTCTTCCcggagcctatcagCAAAGCCATTCCCAAGGCTATGGCTACATGCACCAGACCAGTGTGTCATCCATGAGATCAATGCAGCATTCACCAAATCTAAGGACCTACCGAGCCATGTACGATTACAGTGCCCAGGATGAAGACGAGGTCTCCTTTAGAGACGGCGACTACATCGTCAACGTGCAGCCTATTGACGATGGCTGGATGTACGGCACAGTGCAGAGAACGGGGAGAACAGGAATGCTCCCAGCGAATTACATTGAGTTTGTTaata
MRVPVFEDIKDETEEEKIGEEENEEDQVFYKPVIEDLSMELARKCTELISDIRYKEEFKKSKDKCTFVTDSPMLNHVKNIGAFISEAKYKGTIKADLSNSLYKRMPATIDSVFAGEVTQLQSEVAYKQKHDAAKGFSDYAHMKEPPEVKHAMEVNKHQSNISYRKDVQDTHTYSAELDRPDIKMATQISKIISNAEYKKGQGIMNKEPAVIGRPDFEHAVEASKLSSQIKYKEKFDNEMKDKKHHYNPLESASFRQNQLAATLASNVKYKKDIQNMHDPVSDLPNLLFLDHVLKASKMLSGREYKKLFEENKGMYHFDADAVEHLHHKGNAVLQSQVKYKEEYEKNKGKPMLEFVETPSYQASKEAQKMQSEKVYKEDFEKEIKGRSSLDLDKTPEFLHVKYITNLLREKEYKKDLENEIKGKGMELNSEVLDIQRAKRASEMASEKEYKKDLESIIKGKGMQAGTDTLEMQHAKKAAEIASEKDYKRDLETEIKGKGMQVSTDTLDVQRAKKASEMASQKQYKKDLENEIKGKGMQVSMDIPDILRAKRTSEIYSQRKYKDEAEKMLSNYSTIADTPEIQRIKTTQQNISAVFYKKEVGAGTAVKDSPEIERVKKNQQNISSVKYKEEIKHATAISDPPELKRVKENQKNISNLQYKEQNYKATPVSMTPEIERVRRNQEQLSAVKYKGELQRGTAISDPPELKRAKENQKNISNVYYRGQLGRATTLSVTPEMERVKKNQENISSVKYTQDHKQMKGRPSLILDTPAMRHVKEAQNHISMVKYHEDFEKTKGRGFTPVVDDPVTERVRKNTQVVSDAAYKGVHPHIVEMDRRPGIIVDLKVWRTDPGSIFDLDPLEDNIQSRSLHMLSEKASHYRRHWSRSHSSSTFGTGLGDDRSEISEIYPSFSCCSEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSMRSMQHSPNLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN
維真生物的(de)人源cDNA克隆中,雖然我們大約70%的克隆與RefSeq或GeneBank所(suo)發(fa)表的(de)基因列(lie)序完全一致,但(dan)也(ye)有(you)些克隆含有(you)天然的(de)單核苷酸(suan)多態性而包含一至多個SNPs, 因此與RefSeq或(huo)GeneBank所發(fa)表的列序會(hui)有(you)些不同。我們強烈建議您在購買之前仔(zi)細將我們克隆的(de)序列與您(nin)所要求的(de)序列進行(xing)對(dui)比(bi),以(yi)決定所購買的(de)克隆是否符合您(nin)的(de)要求。如果我(wo)(wo)們預制的(de)克隆不(bu)符合您(nin)的(de)要求,我(wo)(wo)們一般在(zai)1-2周內可(ke)以提供(gong)一(yi)個與(yu)您所要求的(de)序列(lie)完(wan)全一(yi)致的(de)克隆(long)。我們的(de)每一(yi)個克隆(long)與(yu)RefSeq(2013)標準序列相(xiang)比都含(han)有一個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫(ku)會不斷(duan)更新,更新后的標(biao)準序列將來(lai)有可(ke)能(neng)與我(wo)們現(xian)有的克隆序列產生差異。我(wo)們所有的克隆產品(pin)都經過(guo)二代(NextGen)測(ce)序(xu)驗證(zheng),并且保證(zheng)網站(zhan)上克(ke)隆的(de)序(xu)列與您(nin)收到(dao)的(de)克(ke)隆序(xu)列完(wan)全一致。在未經(jing)維真生物書面(mian)給予授(shou)權前,人源ORF cDNA克隆不可轉售或(huo)贈予買方(fang)以外的第三(san)方(fang)。同時(shi), 在使(shi)用任何維(wei)真產品之前,買方有責任(ren)確認該基因的(de)(de)使(shi)用(yong)不會侵犯到第(di)三方的(de)(de)知識(shi)產(chan)權。此產(chan)品專(zhuan)門為基礎(chu)研究所開發,不適(shi)用(yong)于任(ren)何治療或(huo)診斷應用(yong)。