產品名稱:人源cDNA克(ke)隆
產(chan)品規格:500μL甘油菌液
產品運(yun)輸(shu)條件:常溫
產(chan)品儲存條件和期限(xian):-80℃長期保存
產品特點:
? 人源(yuan)基因(yin)C-端融合 Flag & 6×His 標簽;
? 含有puromycin真核抗性基因,可篩選(xuan)穩定轉染細(xi)胞(bao)株;
? 含有卡那霉素(su)原(yuan)核抗(kang)性基(ji)因,可(ke)篩選重組(zu)克隆,推薦使(shi)用濃度為30μg/ml。
產品使(shi)用(yong):
將甘油菌(jun)在新鮮配制(zhi)的(de)固(gu)體培養基(相應抗(kang)性)中劃(hua)線(xian),37℃培(pei)養(yang)(yang)箱培(pei)養(yang)(yang)過夜,挑(tiao)取3個單克(ke)隆,分別接種于相應抗性的液體培(pei)養(yang)(yang)基中,37℃搖(yao)床(chuang)培(pei)養(yang)(yang)過夜,然后進行質粒(li)的提取和(he)驗證。
溫馨提示:考(kao)慮到實驗(yan)的嚴謹性,建議(yi)您(nin)拿到產品的(de)第一時間(jian)進(jin)行測序確認。
ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGACCGTTGGTCCCAGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCCAATGACAGCTCCAAGTTCAAAACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTTAAAGACTTTTCTGGAGACATGTGCAAGCTCAAATGGGTGGAGATTTCTAATGAGGTGAGGAAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGGAACATGTTAAAAATCCTTACAAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGACCCCTTATTTCCGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTGGACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAATATATTCAGGACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGGGAGGATCACCCCGACCTAATCCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAAACCCCCCAGCAGCTGTGGTACACCCACGAGAAGAAGGTGTATCTCAAAGTGCGGCCAGATGCCACTACGAAGGAGGTGAAGGACTCCCTGGGGAAGCAGTGGTCTCAGCTCTCGGACAAAAAGAGGCTGAAATGGATTCATAAGGCCCTGGAGCAGCGGAAGGAGTACGAGGAGATCATGAGAGACTATATCCAGAAGCACCCAGAGCTGAACATCAGTGAGGAGGGTATCACCAAGTCCACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGACGGGCGACCCACCAAGCCACCTCCGAACAGCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAGGACGTGCCCAGCACAGAGCGCATGGTGCTGTGCAGCCAGCAGTGGAAGCTGCTGTCCCAGAAGGAGAAGGACGCCTATCACAAGAAGTGTGATCAGAAAAAGAAAGATTACGAGGTGGAGCTGCTCCGTTTCCTCGAGAGCCTGCCTGAGGAGGAGCAGCAGCGGGTCTTGGGGGAAGAGAAGATGCTGAACATCAACAAGAAGCAGGCCACCAGCCCCGCCTCCAAGAAGCCAGCCCAGGAAGGGGGCAAGGGCGGCTCCGAGAAGCCCAAGCGGCCCGTGTCGGCCATGTTCATCTTCTCGGAGGAGAAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGAGAGCGAGCTGACCCGCCTGCTGGCCCGAATGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAGGCCCGAGAGGCGGCGCTCAAGGCTCAGTCGGAGAGGAAGCCCGGCGGGGAGCGCGAGGAACGGGGCAAGCTGCCCGAGTCCCCCAAAAGAGCTGAGGAGATCTGGCAACAGAGCGTTATCGGCGACTACCTGGCCCGCTTCAAGAATGACCGGGTGAAGGCCTTGAAAGCCATGGAAATGACCTGGAATAACATGGAAAAGAAGGAGAAACTGATGTGGATTAAGAAGGCAGCCGAAGACCAAAAGCGATATGAGAGAGAGCTGAGTGAGATGCGGGCACCTCCAGCTGCTACAAATTCTTCCAAGAAGATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGGTTACCAGAAGTTCTCCCAGGAGCTGCTGTCCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATGGTGGAGATCGGCAGTCGCTGGCAGCGCATCTCCCAGAGCCAGAAGGAGCACTACAAAAAGCTGGCCGAGGAGCAGCAAAAGCAGTACAAGGTGCACCTGGACCTCTGGGTTAAGAGCCTGTCTCCCCAGGACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTAAGAGCATGACCAAGCTGCGAGGCCCAAACCCCAAATCCAGCCGGACTACTCTGCAGTCCAAGTCGGAGTCCGAGGAGGATGATGAAGAGGATGAGGATGACGAGGACGAGGATGAAGAAGAGGAAGATGATGAGAATGGGGACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAGCGAGGACGAGAGCGAGGATGGGGATGAGAATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGACGATGAGGATGAAGATAATGAGTCCGAGGGCAGCAGCTCCAGCTCCTCCTCCTCAGGGGACTCCTCAGACTCTGACTCCAAC
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
維真生物的人源cDNA克隆中,雖然(ran)我(wo)們大約70%的克隆與RefSeq或GeneBank所發表的基因列序完全一致,但也(ye)有些克隆含有天然的(de)單核苷酸多態(tai)性(xing)而包(bao)含一至多個SNPs, 因此與RefSeq或(huo)GeneBank所發(fa)表的列序會有(you)些不同。我們強烈(lie)建議您在購買之前仔細(xi)將我們克隆的(de)(de)序列與您所要求的(de)(de)序列進行對比,以決定(ding)所購買(mai)的克隆(long)是否符(fu)合您的要求。如果(guo)我們預制的克隆(long)不符(fu)合您的要求,我們一般在1-2周內可以提(ti)供一(yi)個與(yu)您所要求的(de)序(xu)列完全一(yi)致(zhi)的(de)克隆(long)。我們的(de)每一(yi)個克隆(long)與(yu)RefSeq(2013)標準(zhun)序(xu)列相比都(dou)含有(you)一個(ge)完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更新(xin)(xin),更新(xin)(xin)后(hou)的標準序列將來有可能與我們現有的克隆(long)序列產生差異。我們所有的克隆(long)產品(pin)都經過二代(dai)(NextGen)測序驗(yan)證(zheng)(zheng),并且(qie)保證(zheng)(zheng)網(wang)站上克隆的(de)(de)序列與(yu)您收到的(de)(de)克隆序列完全一致。在未經維真(zhen)生物書面(mian)給予(yu)授權前(qian),人(ren)源ORF cDNA克隆不可轉售(shou)或贈予買方以外的第三方。同時, 在使用任何維真產品之前,買方(fang)有責任(ren)確(que)認該基因的使(shi)用不會侵犯(fan)到第三方(fang)的知識產權。此產品專門為(wei)基礎(chu)研究所開發,不適用于任(ren)何(he)治療或診斷應(ying)用。