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Ready-to-package AAV shRNA clones，4 predesigned shRNA in pAV-U6-GFP vector + 1 negative control with scrambled sequences

Ready-to-package AAV shRNA clones，4 predesigned shRNA in pAV-U6-GFP vector + 1 negative control with scrambled sequences

基因編號: NM_198856
產品編號: SH896829
基因名稱: EFCAB6; dJ185D5.1; DJBP; FLJ23588; HSCBCIP1; KIAA1672
基因大小: 4050 bp
價格: 3500
促銷價: 3000
供貨時間: 1-2 weeks

  • 產品信息
  • DNA序列
  • siRNAs
  • 免責聲明
產品名稱： 人源shRNA克隆
產品規格： 各500μl甘油菌液+5ug質粒
產品運輸條件：常溫

產品儲存條件和期限：

菌液：-80℃，長期；

質粒：-20℃，長期。

產品特點：

? 帶有GFP，可直觀轉染效率；
? 含有氨芐青(qing)霉(mei)素（Ampicillin ）原核(he)抗性基(ji)因，可篩選重組克隆，推薦使用濃度為50μg/ml。

產品使用：

將甘油菌在新鮮配制的固體培養基（相應抗性）中劃線，37℃培養箱培養過夜，挑取3個單克隆，分別接種于相應抗性的液體培養基中，37℃搖床培養過夜，然后進行質粒的提取和驗證。或者，直接用質(zhi)粒(li)進行轉化。PS：產品質粒濃度在100-200ng/ul之間，若(ruo)需要(yao)更精(jing)準的(de)質粒濃度，請務必(bi)驗(yan)證。

CGCCATGAATTGCTGCCGCACATTAAGAGAACTTGAAATCCAAGTGGGAGAAAAGGTTTTCAAAAACATTAAGACTGTTATGAAAGCCTTTGAGCTCATTGATGTTAACAAGACTGGACTGGTTCGACCGCAGGAGCTAAGAAGGGTTCTGGAGACCTTCTGTATGAAGTTAAGAGACGAGGAATACGAAAAGTTTTCGAAACACTACAACATCCACAAGGATACTGCAGTAGATTACAACGTGTTTTTGAAGAATCTCAGCATAAATAACGACTTGAACCTTAGATATTGTATGGGAAATCAAGAGGTCTCATTGGAGAATCAACAAGCCAAAAATTCCAAAAAGGAACGTTTGCTAGGTTCTGCATCATCTGAAGATATCTGGAGAAACTACTCCTTGGATGAAATTGAGAGGAACTTTTGTCTACAACTTTCGAAGTCTTATGAAAAGGTTGAAAAGGCCCTCAGTGCAGGGGACCCCTGTAAAGGTGGCTACGTGTCTTTTAATTATCTAAAGATTGTCCTCGACACTTTTGTATACCAAATACCAAGAAGAATTTTTATCCAGTTAATGAAAAGATTTGGACTTAAAGCCACCACTAAAATCAATTGGAAGCAATTTCTAACATCATTTCATGAGCCTCAGGGGTTGCAAGTTAGCAGTAAAGGTCCCCTGACAAAAAGAAATAGCATCAACTCTAGAAATGAATCTCACAAGGAAAACATCATCACAAAGTTATTTAGACACACTGAAGATCACTCTGCGTCACTGAAGAAAGCATTACTGATAATCAACACTAAACCCGATGGACCGATAACAAGAGAAGAATTTCGATATATTCTAAATTGCATGGCTGTAAAACTAAGCGATTCAGAATTCAAAGAACTAATGCAAATGCTTGACCCTGGGGACACTGGAGTGGTCAACACCAGCATGTTTATTGATCTGATTGAAGAGAACTGTAGGATGAGAAAGACATCTCCCTGTACAGATGCTAAGACACCTTTCCTCCTGGCCTGGGATTCAGTGGAAGAAATTGTTCATGATACAATTACTAGGAACCTACAAGCTTTTTATAACATGCTACGCTCATATGACCTTGGAGACACAGGGCGCATTGGCCGAAATAATTTCAAGAAAATCATGCACGTCTTCTGTCCATTTTTAACGAATGCACATTTCATAAAACTCTGCAGTAAGATTCAGGACATTGGTTCAGGAAGAATCCTTTACAAGAAACTTTTGGCATGCATAGGAATTGATGGCCCACCCACTGTCTCTCCAGTTCTTGTTCCAAAGGATCAGCTGTTAAGTGAACATTTACAAAAAGATGAACAGCAGCAGCCAGATCTTTCTGAGAGAACCAAGCTCACGGAGGATAAAACCACCCTGACCAAGAAGATGACCACAGAAGAAGTGATTGAAAAATTCAAAAAGTGTATACAGCAGCAGGACCCGGCATTCAAAAAACGATTTCTTGACTTCAGCAAGGAGCCTAATGGAAAAATTAACGTGCATGACTTTAAGAAGGTACTGGAAGACACTGGGATGCCCATGGACGATGATCAGTATGCCCTGCTGACCACTAAAATAGGCTTCGAGAAGGAAGGGATGAGCTATCTTGATTTTGCAGCAGGATTTGAAGATCCTCCAATGAGAGGGCCGGAAACCACTCCGCCGCAGCCTCCAACTCCTTCAAAAAGTTACGTGAACAGCCACTTTATCACCGCAGAAGAATGCCTGAAGCTTTTCCCTAGGAGGCTGAAGGAGTCATTCCGGGACCCCTACTCTGCCTTCTTTAAAACAGATGCTGACAGGGATGGCATAATCAACATGCATGACCTTCACAGACTGCTCCTGCATCTACTGCTTAATCTCAAAGACGACGAGTTTGAGCGCTTCCTTGGCCTTCTTGGCTTGAGACTTAGTGCCACTTTAAATTTTCGGGAATTTCAAAATTTGTGTGAGAAGAGACCATGGAGAACAGATGAAGCGCCTCAAAGACTCATTAGACCAAAACAGAAGGTTGCGGATTCAGAACTAGCTTGTGAGCAGGCTCATCAGTATCTTGTTACCAAAGCAAAAAACAGATGGTCAGACTTGTCTAAGAATTTTCTAGAAACCGATAATGAGGGCAATGGCATTCTTCGACGCCGGGACATAAAGAACGCACTGTACGGTTTTGATATTCCCCTCACACCAAGAGAGTTTGAAAAGCTTTGGGCAAGATACGACACCGAGGGAAAAGGGCACATTACTTACCAGGAATTTTTACAGAAATTGGGTATTAACTATTCGCCTGCTGTCCATCGGCCCTGTGCAGAGGATTATTTCAACTTCATGGGTCATTTTACAAAGCCACAGCAGCTACAGGAAGAGATGAAGGAGCTGCAGCAGAGCACAGAGAAGGCTGTGGCAGCCAGGGATAAGCTTATGGACCGCCATCAAGATATCAGCAAAGCATTCACCAAAACTGATCAATCCAAAACCAACTACATATCCATATGCAAGATGCAGGAAGTGCTGGAAGAATGTGGATGTTCTCTTACCGAAGGGGAGCTGACCCATCTGCTAAACAGTTGGGGAGTCAGCCGGCATGATAATGCTATCAATTACCTCGACTTCCTGAGAGCAGTGGAGAACAGCAAGTCAACAGGAGCTCAGCCCAAGGAAAAAGAAGAGAGCATGCCAATCAATTTTGCAACGCTGAATCCACAGGAGGTTGTGAGGAAGATCCAGGAAGTAGTTGAGTCCTCCCAGCTGGCTTTGTCCACGGCATTTTCTGCATTGGGTAAAGAGGATACAGGATTTGTAAAGGCTACAGAATTCGGACAAGTTCTTAAGGATTTCTGTTACAAACTAACGGACAATCAGTATCATTATTTTTTGAGGAAACTAAGAATTCATCTAACCCCCTATATAAATTGGAAATATTTTCTCCAGAACTTTAGCTGTTTCCTTGAGGAGACAGCTGATGAGTGGGCTGAGAAAATGCCCAAAGGCCCGCCGCCTACCTCTCCCAAGGCCACAGCCGACAGAGACATCCTGGCTCGCCTCCACAAAGCAGTGACTTCCCATTACCATGCCATCACCCAGGAGTTTGAGAATTTTGACACCATGAAAACGAACACCATCTCCAGAGAGGAGTTTAGGGCCATTTGTAATCGCCGCGTCCAAATCCTGACGGACGAACAGTTTGGCAGACTCTGGAACGAGATGCCAGTCAATGCCAAGGGGAGGCTGAAATACCCGGACTTCCTGAGCAGGTTCAGTTCCGAGACAGCAGCCACACCAATGGCCACTGGTGACTCGGCCGTGGCCCAGAGAGGGAGCAGTGTCCCTGACGTCTCGGAAGGCACCAGATCTGCCCTCTCATTGCCCACTCAGGAGCTGAGACCAGGGTCAAAGTCGCAGAGCCACCCCTGTACTCCAGCGAGCACCACCGTGATCCCGGGCACTCCACCCTTGCAGAACTGTGATCCCATAGAGAGCAGGCTCCGGAAGAGAATCCAGGGCTGCTGGCGCCAGCTCCTGAAAGAATGCAAGGAGAAGGACGTGGCCAGACAGGGGGACATCAACGCCTCCGATTTCCTGGCTCTTGTGGAGAAATTCAACCTGGACATAAGCAAAGAGGAGTGTCAGCAGCTCATTATAAAATACGACTTAAAGAGCAACGGGAAATTTGCATACTGTGACTTCATTCAGAGCTGTGTCCTCCTGCTAAAAGCAAAGGAAAGCCCACTGATGCACAGGATGAAGATCCAGAATGCACACAAGATGAAAGAAGCCGGCGCGGAGACGCCATCTTTTTACTCTGCTCTGCTGCGTATTCAGCCCAAAATTGTGCACTGCTGGAGGCCAATGCGGCGCACGTTCAAAAGCTATGATGAGGCTGGAACAGGGCTGCTAAGCGTCGCAGATTTCAGGACGGTCCTGAGACAGTACAGCATCAACCTCTCTGAGGAAGAGTTCTTCCATATTCTGGAGTATTACGATAAGACGCTGTCTTCAAAAATCTCCTACAACGACTTCCTCCGGGCAttcctccaga

siRNA1： AACATTAAGACTGTTATGAAAGC
siRNA2： AAACTAAGCGATTCAGAATTCAA
siRNA3： AAGGAAGGGATGAGCTATCTTGA
siRNA4： AAGATATCAGCAAAGCATTCACC

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